Technical Data
Osteocalcin (Bone-Gla-Protein, BGLAP, BGP, Bone gamma Carboxyglutamate Protein, Gamma Carboxyglutamic Acid-containing Protein, PMF1)
Osteocalcin (Bone Gla Protein) is a 49aa single chain vitamin K dependent protein (MW 5.8kD), made by osteoblasts that is a major component of the non-collagenous bone matrix. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Pagets disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency.

Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:50-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceMouseGoat
Not Determined
Highly purified mouse osteocalcin
Supplied as a liquid, 0.09% sodium azide.
Recognizes intact mouse osteocalcin. Reacts with a site at the carboxyl terminal of Osteocalcin. Does not react with Rat, Human, Bovine, monkey, elk or chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gundberg, C. M. et al. (1992) Development and validation of a radioimmunoassay for mouse osteocalcin: paradoxical response in the Hyp mouse. Endocrinology.130 (4):1909-1915. 2. Matsumoto, T. et al. (2010) Lnk-dependent axis of SCF-cKit signal for osteogenesis in bone fracture healing. J Exp Med. 207: 2207-23. 3. Davies, M.R. et al. (2003) BMP-7 is an efficacious treatment of vascular calcification in a murine model of atherosclerosis and chronic renal failure. J Am Soc Nephrol. 14 (6): 1559-67. 4. Tasso, R. et al. (2009) Recruitment of a host's osteoprogenitor cells using exogenous mesenchymal stem cells seeded on porous ceramic. Tissue Eng Part A. 15: 2203-12. 5. Qian, H. et al. (2013) Molecular characterization of prospectively isolated multipotent mesenchymal progenitors provides new insight into the cellular identity of mesenchymal stem cells in mouse bone marrow. Mol Cell Biol. 33: 661-77. 6. Cherry, H.M. et al. (2014) In vivo phenotypic characterisation of nucleoside label-retaining cells in mouse periosteum. Eur Cell Mater. 27: 185-95; discussion 195.