Technical Data
O8060-25A-APC
Osteocalcin, NT (Gamma-carboxyglutamic Acid-containing Protein, Bone Gla-protein, BGP, BGLAP) (APC)
Description:
Prior to the formation of calcified bone, noncollagenous proteins form in the extracellular bone matrix. Gamma-carboxyglutamic acid residues are formed by vitamin K, vitamin-D regulated calcium binding proteins containing residues of Gla. These residues are essential for the binding of calcium and constitue 1-2% of total bone protein. Osteocalcin itself binds strongly to apatite and calcium. Production of osteocalcin is expressed late in normal bone development and is characteristic of mature osteoblasts. Regular osteocalcin production has been shown to be linked to the p53 tumor suppressor gene. The p53 gene undergoes rearrangement in a high percentage of osteosarcomas, resulting in loss of its expression. The loss of p53 regulation inhibits further osteocalcin production. The absence of end-point differentiation in bone due to p53 rearrangements and lack of osteocalcin production may contribute to the maintenance of the tumorigenic phenotype in osteosarcomas.

Applications:
Suitable for use in FLISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
FLISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the N-terminal region of human osteocalcin (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).
Specificity:
Recognizes human Osteocalcin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Yamada, Y., et al., J. Clin. Endocrinol. Metab. 88(7):3372-3378 (2003). 2.Gronthos, S., et al., J. Bone Miner. Res. 18(4):716-722 (2003). 3.Yousfi, M., et al., Biochem. Biophys. Res. Commun. 297(3):641-644 (2002). 4.Willis, D.M., et al., J. Biol. Chem. 277(40):37280-37291 (2002). 5.Viereck, V., et al., J. Cell. Biochem. 86(2):348-356 (2002).