Technical Data
Osteocalcin, NT (Bone-Gla-Protein, BGP)
Osteocalcin, also known as Bone-Gla-Protein (BGP), is a single chain (Mr=5800) vitamin K dependent protein produced by osteoblasts. It is found in high concentrations in bone. Post-translational modification by a vitamin K-dependent carboxylase produces three g-carboxyglutamic acid residues at positions 17, 21 and 24. The mature protein contains 49 amino acids and a single intrachain disulfide bond joining Cys23 to Cys29. The secondary structure is highly calcium dependent and contains 14% alpha-helix, 20% beta-sheet and 67% random form in the presence of calcium, and 1% alpha-helix, 20% beta-sheet and 79% random form in the absence of calcium. BGP binds to phospholipid vesicles in the presence of calcium ions (Kd=6x10e-6M). BGP also binds hydroxyapatite and is an efficient inhibitor of hydroxyapatite seeded crystal growth, suggesting a regulatory role in bone mineralization. Although the fundamental role of BGP in vivo remains elusive, in vitro experimentation suggests roles in both bone resorption and bone formation. BGP has been shown to function as a chemo-attractant in an in vitro macrophage mediated bone resorption system. The serum concentration of BGP has been linked to bone formation rates, and BGP levels are currently used in research assessment of osteoporosis and other metabolic bone disease states.

Suitable for use in Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1-2ug/ml. after Trypsin treatment Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceMouseRabbit
~2mg/ml (after reconstitution)
Synthetic peptide corresponding to aa1-20, YLGASVPSPDPLEPTRE- QCE of intracellular N-terminal mouse Osteocalcin (P86547).
Purified by Protein A and immunoaffinity chromatography.
Supplied as a lyophilized powder from 10mM PBS, pH 7.4, 1% BSA. Reconstitute with 50ul sterile ddH2O, 0.1 sodium azide. Dilute further with 10mM PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Recognizes intracellular N-terminal mouse Osteocalcin. Does not crossreact with rat Osteocalcin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Price, P.A., et al., in Bone and Mineral Research, Vol. 1, ed. Peck, W., pp. 157-190, Ansterdam: Escerpta Medica (1983).2. Hauschka, P.V., et al., PNAS USA 73: 1447 (1975).3. Gundberg, C.M., et al., Methods Enzymol. 107: 516 (1984).4. Delmas, P.D., et al., Biochemistry 23: 4720 (1984).5. Gendreau, M.A., et al., J. Biol. Chem. 264: 6972 (1989). 6. Romberg, R.W., et al., Biochemistry 25: 1176 (1986).7. Tracy, R.P., et al., J. Bone Min. Res. 5: 451 (1990). 8. Romberg, R.W., et al., J. Biol. Chem 260: 2728 (1985). 9. Villarreal, X.C., et al., Biochemistry 28: 6483 (1989). 10. Kelm, R.J., et al., Blood 80: 3112 (1992).