Technical Data
O8061-18G
Osteocrin (OSTN, Musclin)
Description:
Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4kD. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures. Treatment of cultures with 1,25-dihydroxyvitamin D3 resulted in a rapid dose-dependent down-regulation of osteocrin expression, suggesting direct regulation. Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression. These results suggest that osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin-fixed paraffin): 1:50 (ABC, DAB)
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat/mouse heart tissue

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to aa31-78 of human Musclin.
Purity:
Purified
Form
Supplied as a lyophilized powder. Reconstitute with sterile dH2O.
Specificity:
Recognizes human Musclin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.