Technical Data
O8063-04
Osteonectin (SPARC)
Description:
SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid , 43 kD protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs . SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/ml
ELISA: 1:4000 (0.5ug/ml)
Western Blot: 5-10ug/ml; Reducing or non-reducing conditions, EDTA-extracted fraction of bovine bone.
Immunohistochemistry: 5-10ug/ml (human cartilage tissue)
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma cell line P3-X63-Ag8-U1 with BALB/c mouse spleen cells.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13G221Highly Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceBovineMouse
Concentration:
~2mg/ml (after reconstitution)
Immunogen:
Bovine Osteonectin
Purity:
Purified by ammonium sulfate fractionation and ion-exchange chromatography from mouse ascites fluid.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.1% sodium azide. Reconstitute with 50ul sterile ddH2O.
Specificity:
Recognizes bovine Osteonectin. Specific for bone-derived and platelet-derived proteins. Reacts with thrombin-stimulated platelet in Flow Cytometry. Does not react with non-activated platelet. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sage, E.H. & Bornstein, P., J. Biol. Chem. 266: 14,831–14,834 (1991). 2. Termine, J.D., et al., Cell 26: 99–105 (1981). 3. Breton-Gorius, J., et al., Blood 79: 936–941 (1992). 4. Kamihagi, K., et al., Biochem. Biophy. Res. Comm. (1994) (in press).