Technical Data
Osteonectin, aa272-286 (ON, Basement Membrane Protein 40, BM40, BM-40, Secreted Acidic Cysteine-rich Glycoprotein, Secreted Protein Acidic and Rich in Cysteine, Secreted Protein Acidic Cysteine-rich, SPARC, SPRC)
SPARC, also known as Osteonectin and basement membrane protein BM-40, is a phosphorylated glycoprotein with calcium-binding domains. It is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC may be involved in cell proliferation, repair of tissue damage and morphogenetic processes such as modeling of extracellular matrix.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:3000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
20ul-20CBlue IceHumanRabbit
Not Determined
Synthetic peptide corresponding to aa272-286, CFGIKQKDIDKDLVI, from human Osteonectin (GeneID 6678, Swiss-Prot P09486).
Supplied as a lyophilized powder. Reconstitute with 20ul or 100ul sterile ddH2O.
Recognizes synthetic human Osteonectin (aa272-286), and recombinant human Osteonectin. Does not crossreact with human Osteopontin or human Bone Sialoprotein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.