Technical Data
O8063-05B
Osteonectin, CT (SPARC, Secreted Protein Acidic And Rich In Cysteine, ON, Basement-membrane Protein 40, BM-40, ON)
Description:
Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM).

Applications:
Suitable for use in Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:10-1:50
Western Blot: 1:1000
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to a region between aa 224-251 from the C-terminal region of human SPARC, conjugated to KLH. Species sequence homology: bovine, chicken, mouse, porcine, rat, rabbit, xenopus
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human SPARC.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Said,N., Oncogene 28(39), 3487-3498 (2009). 2. Maloney,S.C., Anticancer Res. 29(8), 3059-3064 (2009).