Technical Data
Osteopontin, aa75-90 (OPN, Bone Sialoprotein I, BSP-1, Secreted Phosphoprotein 1, SPP1, Early T-Lymphocyte Activation 1)
Osteopontin is a single-chain polypeptide with a molecular weight of ~35kD. It is a glycoprotein first identified in osteoblasts. It is biosynthesized by a variety of tissue types including preosteoblasts, osteoblasts, osteocytes, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta, odontoblasts, some bone marrow cells, hypertrophic chondrocytes, macrophages, smooth muscle, and endothelial cells. Osteopontin is overexpressed in a variety of cancers, including lung cancer, breast cancer, colorectal cancer, stomach cancer, ovarian cancer, melanoma and mesothelioma. It may contribute to kidney stone formation and both glomerulonephritis and tubulointerstitial nephritis and is also found in atheromatous plaques within arteries. Research has implicated osteopontin in excessive scar-forming and a gel has been developed to inhibit its effect.

Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Immunohistochemistry (Paraffin): 1:1500
Western Blot: 1:500 detected Osteopontin in 10ug of RAW 264.7 lysates.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human urine (2-5ug/ml normal concentration)

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

Storage and Stability:
May be stored at 4░C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20░C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20░CBlue IceHumanRabbit
Not Determined
Synthetic peptide corresponding to aa75-90, PSKSNESHDHMDDMDD from human osteopontin (P10451). Species Sequence Homology: rat (88%)
Supplied as a liquid. No preservatives added.
Recognizes human osteopontin. No crossreactivity with human osteonectin or human bone sialoprotein II. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.áManzano, V.M., et al., British Journal of Pharmacology 131: 1673-1683 (2000). 2. Tezuka, K., et al., Biochem. Biophys. Res. Commun. 186: 911-917 (1992).