Technical Data
Osteoprotegerin (OPG, Osteoclastogenesis Inhibitory Factor, OCIF)
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization. Other applications not tested.

Recommended Dilution:
indirect ELISA: 0.5-2ug/ml. Using 100ul/well, used in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.53.0ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry: 0.5ug/ml on formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. Incubate overnight at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH of 6.0 sodium citrate buffer is recommended.
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
Highly pure (>98%) recombinant human OPG (human Osteoprotegerin).
Puri ed by Immunoaf nity chromatography.
Supplied as a liquid in PBS, pH 7.2.
Recognizes human OPG.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.