Technical Data
O8065-03
Osteoprotegerin (OPG, Osteoclastogenesis Inhibitory Factor, OCIF) (Biotin)
Description:
Osteoprotegerin (OPG, or osteoclastogenesis inhibitory factor) is a secretory glycoprotein belonging to TNF receptor superfamily. OPG consists of 401 amino acid with molecular weight of 55kD as a monomer and 110kD as a disulfide-linked dimer. Osteoprotegerin acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis associated with increased osteoclast activity.

Applications:  
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
Direct ELISA: 0.25-1ug/ml (100ul/well) Detects at least 0.2-0.4ng/well of recombinant hOPG when used with compatible secondary reagents.
Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant hOPG is 1.5-3.0ng/lane, under either reducing or non-reducing conditions when used with compatible secondary reagents.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.1% BSA. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
0.1mg/ml
Immunogen:
Highly pure (>98%) recombinant hOPG (human OPG).
Purity:
Puri ed by af nity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Labeled with Biotin. Reconstitute with 500ul 40-50% glycerol and sterile PBS containing 0.1% BSA.
Specificity:
Recognizes recombinant hOPG.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.