Technical Data
Osteoprotegerin (OPG, Osteoclastogenesis Inhibitory Factor, OCIF) (Biotin)
Osteoprotegerin (OPG, or osteoclastogenesis inhibitory factor) is a secretory glycoprotein belonging to TNF receptor superfamily. OPG consists of 401 amino acid with molecular weight of 55kD as a monomer and 110kD as a disulfide-linked dimer. Osteoprotegerin acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis associated with increased osteoclast activity.

Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
Direct ELISA: 0.25-1ug/ml (100ul/well) Detects at least 0.2-0.4ng/well of recombinant hOPG when used with compatible secondary reagents.
Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant hOPG is 1.5-3.0ng/lane, under either reducing or non-reducing conditions when used with compatible secondary reagents.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.1% BSA. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
25ug4C (-20C Glycerol)Blue IceHumanRabbit
Highly pure (>98%) recombinant hOPG (human OPG).
Puri ed by af nity chromatography.
Supplied as a lyophilized powder in PBS, pH 7.2. Labeled with Biotin. Reconstitute with 500ul 40-50% glycerol and sterile PBS containing 0.1% BSA.
Recognizes recombinant hOPG.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.