Technical Data
8-Oxoguanine (8-Hydroxyguanine)
8-Oxoguanine is a mutagenic oxidative damage product of guanine. Oxidatively damaged base 8-oxoguanine is generated in the DNA of all living organisms due to the presence of reactive oxygen species in cells. DNA damage threatens the health of the genetic information stored in every DNA molecule; however enzymes exist in cells to protect against the mutagenic effect of this lesion. 8-oxoguanine is one of the most abundant and well-characterized DNA lesions generated by oxidative stress. It has been estimated that ~180 guanines are oxidized to 8-oxoG per mammalian cell per day. 8-oxoG is a miscoding lesion that can cause G:C to T:A or T:A to G:C transversion mutations. This lesion accumulates in DNA with age and it has been loosley linked to several cancers and diseases such as Alzheimer's and Parkinson's.

Suitable for use in ELISA and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: Detects 8-Oxoguanine in HeLa and Cos7 cells fixed with paraformaldehyde. 8-Oxoguanine has been localized to the nucleus in nutrient-deprived cells.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Nutrient starved HeLa cells or Cos7 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceMouse
Not Determined
8-oxoguanine adsorbed onto alumina
Supplied as a liquid. No preservative added.
Recognizes 8-oxoguanine. Exhibits reactivity with dGMP, dAMP, dCMP or TMP in millimolar, but not in micromolar concentrations. Species Crossreactivity: human, rat, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Conlon, K., et al., J. Histotechnology 23: 37-44 (2000).