Technical Data
P1000-54X
p21, WAF1 (Cip1, Sdi1, Pic1,Wild-type p53-activated Fragment, Tumor Suppressor Protein) (BSA & Azide Free)
Description:
Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21WAF1. p21WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation and Immunohistology. Not suitable for Flow Cytometry or Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (native and denatured) : 2ug/ml protein lysate. Co-precipitates p21 proteins.
Immunohistochemistry (formalin and paraffin): 1:25-1:50 for 60 minutes at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Ls174T or Raji cells and colon carcinoma.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a0.N.488Affinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Full length human recombinant p21 protein. MW of Antigen: 21kD Cellular Localization: Nuclear.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also supplied with BSA and sodium azide. See P1000-54.
Specificity:
Recognizes human p21WAF1/Cip1/Sdi1/Pic1 tumor suppressor protein. Does not react with mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.