Technical Data
P1000-55X
p21, WAF1 (Cip1, Sdi1, Pic1,Wild-type p53-activated Fragment, Tumor Suppressor Protein) (BSA & Azide Free)
Description:
Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21WAF1. p21WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry (formalin/paraffin), and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 2-4ug/ml for 30 minutes at room temperature.
Immunoprecipitation: 2ug/mg protein lysate. Co-precipitates cdk4.
[Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.]
Optimal dilutions to be determined by the researcher.

Positive Control:
Ls174T or Raji cells and colon carcinoma.

Cellular Localization:
Nuclear

Molecular Weight of Antigen:
21kD

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.489Affinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Full length human recombinant p21 protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also supplied with BSA and sodium azide. See P1000-55.
Specificity:
Recognizes Human, bovine, Mouse and Rat p21WAF1/Cip1/Sdi1/Pic1 tumor suppressor protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.