Technical Data
P1001-24E
p47 phox (NCF1, NOXO2, Neutrophil cytosolic factor 1, 47kD Chronic granulomatous disease, autosomal 1)
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Technical Data |
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P1001-24E |
p47 phox (NCF1, NOXO2, Neutrophil cytosolic factor 1, 47kD Chronic granulomatous disease, autosomal 1) |
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Description: Neutrophil cytosolic factor 1 (NCF1), a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 0.2-2ug/ml. Detects a band of approximately 47kD in U937 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody. Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-61G IgG H&L, X-adsorbed (HRP) DkxSh Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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