Technical Data
P1001-24E
p47 phox (NCF1, NOXO2, Neutrophil cytosolic factor 1, 47kD Chronic granulomatous disease, autosomal 1)
Description:
Neutrophil cytosolic factor 1 (NCF1), a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.2-2ug/ml. Detects a band of approximately 47kD in U937 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-61G IgG H&L, X-adsorbed (HRP) DkxSh

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptie sequence CSESTKRKLASAV corresponding to the C-terminus of NCF1 (NP_000256).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.3, 0.02% sodium azide, 0.5% BSA.
Specificity:
Recognizes neutrophil cytosolic factor 1 (NCF1). Species Crossreactivity: Human. Species sequence homology: dolphin, bovine, mouse, rabbit, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Davidson-Moncada, J. K. et al. (2002). Involvment of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils. Biochem. J. 363: 95-103.

PMID: 001903052.

LocusLink ID - 468779444.




GenBank