Technical Data
P1001-24J
p47 phox (NCF1, NOXO2, p47phox, neutrophil cytosolic factor 1, 47kD, chronic granulomatous disease, autosomal 1, Neutrophil cytosolic factor-1 (47kD)
Description:
Neutrophil cytosolic factor 1 (NCF1) is a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.2-2ug/ml~47kD band seen in U937 cell lysates.
Optimal dilution to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See P1001-24J-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanGoat
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide CSESTKRKLASAV, corresponding to C Terminus of human p47 phox.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide before the addition of glycerol to 40%.
Specificity:
Recognizes Human NCF1/p47phox. Species Sequence Homology: Mouse, rat, rabbit, bovine .
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Davidson-Moncada JK, Lopez-Lluch G, Segal AW, Dekker LV, Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils., Biochem J 2002 Apr 1;363(Pt 1):95-103