Technical Data
P1001-37S1
p70 S6 kinase
Description:
p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunohistochemistry (Paraffin): 1:50
Immunoprecipitation: 1:60
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgGSupernatant
SizeStorageShippingSourceHost
100ul -20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to residues surrounding human p70 S6 kinase.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human p70 S6 kinase. Species crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pearson, R.B. and G. Thomas. Regulation of p70s6k/p85s6k and its role in the cell cycle. Prog Cell Cycle Res. 1: 21-32 (1995). 2. Pullen, N. and G. Thomas. The modular phosphorylation and activation of p70s6k. FEBS Lett. 410: 78-82 (1997). 3. Dufner, A. and G. Thomas. Ribosomal S6 kinase signaling and the control of translation. Exp Cell Res. 253: 100-109 (1999). 4. Le, X.F., et al. Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis. Oncogene 22: 484-497 (2003).