		Technical Data
P1699-76B	PA11S (Proteasome Activator 11S Subunit REG gamma, Proteasome Activator 11S REG gamma, 11S Regulator Complex gamma Subunit, Activator of Multicatalytic Protease Subunit 3, Ki, Ki Antigen, Ki Nuclear Autoantigen, Ki, Proteasome (Prosome, Macropain) Activat

Description:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified.

Positive Control: K562 cell lysate

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide, C-KNIEKIKRPRSSN, representing the C Terminus of the sequence according to NP_005780.2; NP_789839.1. Homology: Human, Mouse, Rat, Dog Epitope: KNIEKIKRPRSSN

Purity:

Purified by immunoaffinity chromatography.

Form

Tris-saline [pH7.3] with 0.5% BSA

Specificity:

Species Crossreactivity: Human. Other species not tested. Expected based upon sequence homology: Mouse, Rat, canine

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Matsudaira R, et al. Relationships between autoantibody responses to deletion mutants of Ki antigen and clinical manifestations of lupus. J Rheumatol. 2003 Jun;30(6):1208-14. PMID: 12784391