Technical Data
P1951-30
PAG3, phosphorylated (Tyr724) (Paxillin-associated Protein with ADP-ribosylation Factor GTPase-activating Protein 3)
Description:
PAG3 (paxillin-associated protein with ADP-ribosylation factor [ARF] GTPase-activating protein GAP] activity, number 3) is a member of the ARF GAP family of proteins that associates with the cytoskeleton, plays an important role in mediating actin cytoskeletal organization in response to adhesion and growth factor stimulation and is involved in paxillin subcellular localization. During monocyte maturation, the elevation of paxillin expression and integrin activation is associated with the induction of PAG3 expression. Overexpression of PAG3 inhibits paxillin recruitment to focal contacts and hinders cellular migration. PAG3 is phosphorylated by Pyk2 kinase on tyrosines 724 and 763.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
COS cells overexpressing human wild-type PAG3, cotransfected with mouse Pyk2.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
10Blots-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human PAG3 that contains tyrosine 724.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity:
Recognizes human PAG3 when phosphorylated at Tyr724. Species sequence homology: mouse (92%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wade, R., et al. (2002) Paxillin null embryonic stem cells are impaired in cell spreading and tyrosine phosphorylation of focal adhesion kinase. Oncogene. 21(1):96-107. 2. Turner, C.E., et al. (2001) Paxillin-ARF GAP signaling and the cytoskeleton. Curr. Opin. Cell. Biol. 13(5):593-599. 3. Uchida, H., et al. (2001) PAG3/Papalpha/KIAA0400, a GTPase-activating protein for ADPribosylation factor (ARF), regulates ARF6 in Fcgamma receptor-mediated phagocytosis of macrophages. J. Exp. Med. 193(8):955-966. 4. Kondo, A., et al. (2000) A new paxillin-binding protein, PAG3/Papalpha/KIAA0400, bearing an ADP-ribosylation factor GTPase-activating protein activity, is involved in paxillin recruitment to focal adhesions and cell migration. Mol. Biol. Cell. 11(4):1315-1327.