Technical Data
PIAS4, CT (E3 SUMO-protein Ligase PIAS4, Protein Inhibitor of Activated STAT Protein 4, Protein Inhibitor of Activated STAT Protein gamma, PIAS-gamma, PIASy, PIASG)
PIASy1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway, the wnt pathway and the steroid hormone signaling pathway. PIASy1 is involved in gene silencing, and promotes PARK7 sumoylation.

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
Synthetic peptide selected from the C-terminal region of human PIASy1 (KLH).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human PIASy1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Imoto, S., et al., J. Biol. Chem. 278(36):34253-34258 (2003). 2. Chun, T.H., et al., Circ. Res. 92(11):1201-1208 (2003). 3. Subramanian, L., et al., J. Biol. Chem. 278(11):9134-9141 (2003). 4. Liu, B., et al., Proc. Natl. Acad. Sci. USA 98(6):3203-3207 (2001). 5. Liu, B., et al., Proc. Natl. Acad. Sci. USA 95(18):10626-10631 (1998).