Technical Data
P2150-70
Paired Immunoglobulin-Like Type 2 Receptor-Ligand (PILR-L, CD99, MIC2)
Description:
PILR-L (also known as CD99 and MIC2) is a 125 amino acid, 24-30 kD glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. PILR-L has been implicated in processes like homotypic cell adhesion, diapedesis and oncosupression. The extracellular domain of the mouse PILR-L protein used as immunogen is 45% identical to the human counterpart.

Applications:
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse PILR-L. The detection limit for rmPILR-L is approximately 2 ng/lane under non-reducing and reducing conditions, respectively.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse PILR-L. The detection limit for rmPILR-L is approximately 0.1 ng/well. In this format, this antibody shows less than 1% crossreactivity with rmPILR- and rmPILR- .
Optimal dilutions should be determined by each laboratory for each application.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseGoat
Concentration:
As reported
Immunogen:
NS0-derived rmPILR-L (aa25-138)
Purity:
Purified by mouse PILR-L affinity chromatography.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity:
Selected for its ability to recognize mouse PILR-L in direct ELISAs and Western Blots.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.