Technical Data
P2488
PAK1 (p21 GTPase activated protein kinase 1)
Description:
The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase and growth factor- induced neurite outgrowth. Several mechanisms that induce PAK activation have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain at the N-terminal region of PAK causes autophosphorylation and conformational change of PAK. Phosphorylation of PAK1 at threonine 423 by PDK induces activation of PAK1. Several autophosphorylation sites have been identified, including serines 199 and 204 of PAK1 and serines 192 and 197 of PAK2. Because the auto- phosphorylation sites are located in the N-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation. Recent research indicates that phosphorylation of serine 144 of PAK1 or serine 139 of PAK3, which is located in the kinase inhibitory domain, affects the kinase activity. Phosphorylation of serine 21 of PAK1 or serine 20 of PAK2 regulates its binding with the adaptor protein Nck. PAK4, PAK5 and PAK6 have lower sequence similarity with PAK13 in the regulatory N-terminal region. It has been demonstrated that phosphorylation of serine 474 of PAK4, an analogous site to threonine 423 of PAK1, may play a pivotal role in the activity and function of PAK4 kinase.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide derived from the N-terminal sequence of human PAK1 (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity:
Detects endogenous levels of total human PAK1 at~68kD. Does not crossreact with PAK2, PAK3 or other PAKs. Species Crossreactivity: monkey, mouse and rat, guinea pig.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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