Technical Data
P3105-07
Papilloma Virus, Human, Type 16, Early Protein 7 (hPV)
Description:
Specific types of HPV have been shown to be associated with human cervical cancer. One of these types, HPV16, has been shown to be present in the majority of cervical dysplasias and squamous cell carcinomas studied. Research has also shown that the expression of the HPV16-E7 gene is both sufficient and necessary for cellular transformation in vitro. The immunological detection of HPV types in human samples has been hindered by the lack of an in vitro culture system for the propagation of viruses and viral antigens. Although antibodies that were generated against the bovine papillomavirus capsid antigen (BPV-L1 ORF) are cross-reactive to HPV, these antibodies are of limited use because they do not discriminate among HPV types and have limited sensitivity as compared to standard light microscopic criteria for HPV infection. With bacterial expression systems, fusion proteins of HPV-encoded gene products can be generated and these, in turn, used to develop HPV type specific antibodies. The use of these type specific antibodies to study HPV protein expression may expand understanding of the role of HPV proteins in the pathogenesis of condylomas, dysplasias, and carcinomas of the genital tract.

Applications:
Suitable for use in ELISA, Flow Cytometry and Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control:
Cervical tissue infected with HPV-16

Cellular Localization:
Nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k0.N.332Affinity Purified
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Recombinant HPV-16 E7 protein
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide before the addition of glycerol to 40%. Also available without BSA and azide. See cat#P3105-07X.
Specificity:
Reacts with a 15kD protein, identified as E7 early protein of type 16 human papilloma virus (HPV 16-E7).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tommasino, M., et al., Oncogene, 8: 195-202, (1993).