Technical Data
P3105-07A
Human Papilloma Virus Type 16 E7 (HPV)
Description:
Human papillomavirus (HPV) is a diverse group of DNA-based viruses that infect skin and mucous membranes of humans and animals. Some HPV types are the causative agents of cervical cancer, with types 16 and 18 being particularly high-risk. The viral proteins E6 and E7 disrupt normal cell cycle regulation by interacting with p53 (a tumor-suppressing transcription factor) and Rb (retinoblastoma protein, also a tumor-supressor). E7 particularly binds to Rb and histone deacetylases, resulting in activation of the E2F genes, which code for a family of transcription factors. The viral proteins E6 and E7 might be of particular interest in the development of therapeutic vaccines, since they are expressed early in viral infection.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Suitable for use as the capture or solid phase antibody.
Matched to P3105-16D, P3105-16F or P3105-25C as the detection or conjugate (label) antibody.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a9B102Affinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceMouse
Concentration:
~2mg/ml
Immunogen:
HPV oncoprotein E7, type 16
Purity:
Purified by Protein G chromatography. >95% (SDS-PAGE).
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes Human Papilloma Virus Type16 (HPV16) E7 Protein. Crossreacts with HPV Type 18. Reacts with the monomer and dimer forms when tested in Western Blot.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.