Technical Data
P3105-07N
Papilloma Virus, Human, Type 16, E1, E4 (hPV, ATP-dependent Helicase E1, E1, E4, Human Papilloma Virus Type 16 ATP-dependent Helicase E1, Human Papillomavirus Type 16 ATP-dependent Helicase E1, HPV16 ATP-dependent Helicase E1, HPV-16 ATP-dependent Helicas
Description:
This antibody is of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.

Cellular Localization: Nuclear

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19A269Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Fusion protein: PWAPKK, corresponding to amino acids 36-41 of HPV16 E1+E4. Antigen for hybridoma production was expressed as a b galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide. Ep
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Recognizes the human papilloma virus type 16 protein. Species Crossreactivity: Human papilloma virus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Doorbar J et al. The E1E4 protein of human papillomavirus type 16 associates with a putative RNA helicase through sequences in its C terminus. J Virol 74:10081-95 (2000). Doorbar J et al. Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein. Virology 187:353-9 (1992).