Technical Data
Papilloma Virus, Human, Type 16, E1, E4 (hPV, ATP-dependent Helicase E1, E1, E4, Human Papilloma Virus Type 16 ATP-dependent Helicase E1, Human Papillomavirus Type 16 ATP-dependent Helicase E1, HPV16 ATP-dependent Helicase E1, HPV-16 ATP-dependent Helicas
HPV16 is an ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans. This antibody is of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.

Suitable for use in Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceMouse
Human Fusion protein. Antigen for hybridoma production was expressed as a b-galactosidase fusion protein using the pEX expression system and was consequently cleaved to release the E1/E4 polypeptide. Cellular Localization: Nuclear.
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Recognizes HPV16 E1+E4. Epitope: E1/E4 protein is aa36-41 of human papilloma virus type 16.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Doorbar, J., et al., J. Virol. 74: 10,081-10,095 (2000). 2. Doorbar, J., et al., Virology 187: 353-359 (1992).