		Technical Data
P3105-08A	Papilloma Virus, Human, Type 16 L1, Late Protein (hPV)

Description:
Specific types of HPV have been shown to be associated with human cervical cancer. One of these types, HPV16, has been shown to be present in the majority of cervical dysplasias and squamous cell carcinomas studied. Research has also shown that the expression of the HPV16-E7 gene is both sufficient and necessary for cellular transformation in vitro.

Applications:
Suitable for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: paraffin embedded, acetone or paraformaldehyde fixed sections are suitable.
Western Blot: detects a 56kD band corresponding to the L1 protein. It also detects a 41kD protein in uninfected mammalian cells. This unwanted specificity is absent in IPPT/IHC.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	10F267	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~1mg/ml

Immunogen:

aa198-531 of HPV 16-L1 fused to beta-galactosidase.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes a late protein of HPV type 16. Detects HPV in human biopsies and smears. HPV-6 and -11 positive biopsies gave negative results.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Browne, H. M. et al. (1988) Analysis of the L1 gene product of human papillomavirus type 16 by expression in a vaccinia virus recombinant J. Gen. Virol. 69: 1263-1273.