Technical Data
Papilloma Virus, Human, Type 16,18 E6 (hPV, Human Papillomavirus Type 16 E6, HPV16 E6, Human Papillomavirus Type 18 E6, HPV18 E6, Protein E6)
Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1, 6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumors of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation. Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.

Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: Reveals a 16.5kD MW polypeptide in cells derived from human cervical carcinoma.
Immunohistochemistry: formalin fixed paraffin sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceMouse
Gel-purified HPV-18 E6-beta galactosidase fusion protein.
Supplied as a liquid in PBS.
Recognizes human Papilloma Virus Type 16 and Papilloma Virus Type 18 E6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Banks L et al. Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas. J Gen Virol 68 ( Pt 5):1351-9 (1987). 2. Kobayashi A et al. Lymphoid follicles are generated in high-grade cervical dysplasia have differing characteristics depending on HIV status. Am J Pathol 160:151-64 (2002). 3. Abdulkarim B et al. Antiviral agent Cidofovir restores p53 function enhances the radiosensitivity in HPV-associated cancers. Oncogene 21:2334-46 (2002).