Technical Data
P3105-15A
Papilloma Virus, Human, Type 16 Capsid L1 (hPV)
Description:
Specific types of HPV have been shown to be associated with human cervical cancer. One of these types, HPV16, has been shown to be present in the majority of cervical dysplasias and squamous cell carcinomas studied. Research has also shown that the expression of the HPV16-E7 gene is both sufficient and necessary for cellular transformation in vitro. The immunological detection of HPV types in human samples has been hindered by the lack of an in vitro culture system for the propagation of viruses and viral antigens. Although antibodies that were generated against the bovine papillomavirus capsid antigen (BPV-L1 ORF) are cross-reactive to HPV, these antibodies are of limited use because they do not discriminate among HPV types and have limited sensitivity as compared to standard light microscopic criteria for HPV infection. With bacterial expression systems, fusion proteins of HPV-encoded gene products can be generated and these, in turn, used to develop HPV type specific antibodies. The use of these type specific antibodies to study HPV protein expression may expand understanding of the role of HPV proteins in the pathogenesis of condylomas, dysplasias, and carcinomas of the genital tract.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin; fixation with acetone or paraformaldehyde is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k9L396Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Beta galactosidase-L1 fusion protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes HPV Type 16, L1 (major capsid protein) at ~41kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Karem, K.L., et al., (2002), "Optimization of a Human Papillomavirus-Specific Enzyme-Linked Immunosorbent Assay", Clinical and Diagnostic Laboratory Immunology, 9(3): 577-582.