Technical Data
P3105-16B
Papilloma Virus, Human, Type 16 Capsid L1 (hPV, HPV-16, HPV-16 Capsid, HPV16 Capsid Protein, Human Papillomavirus Type 16 L1, HPV16 L1, Human Papillomavirus Type 16 Major Capsid Protein L1, HPV16 Major Capsid Protein L1, Major Capsid Protein, Major Capsid
Description:
L1 is a major capsid protein of type 16 human papilloma virus. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a9A275Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Human papilloma virus type 16, major capsid protein L1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS.
Specificity:
Recognizes Papilloma Virus, Human, Type 16 Capsid L1. Reacts with a 56kD protein in cells infected with L1-vaccinia virus, the protein being present in a predominantly nuclear location. Reacts very strongly with biopsy specimins containing HPV-16 or -33.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McLean, C.S., et al., Production characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus. J. Clin. Pathol. 43: 488-492 (1990). 2. Zhang, W., et al., Expression of human papillomavirus type 16 L1 protein in Escherichia coli: denaturation, renaturation, self-assembly of virus-like particles in vitro. Virology 243: 423-431 (1998).