Technical Data
Papilloma Virus Type 16 E7 Protein (E7, HPV16 E7, HpV16gp2, Links Transforming Protein Human Papillomavirus 16, Protein E7, Transforming Protein, Transforming Protein Human Papillomavirus 16)
Human papillomavirus (HPV) is a diverse group of DNA-based viruses that infect skin and mucous membranes of humans and animals. Some HPV types are the causative agents of cervical cancer, with types 16 and 18 being particularly high-risk. The viral proteins E6 and E7 disrupt normal cell cycle regulation by interacting with p53 (a tumor-suppressing transcription factor) and Rb (retinoblastoma protein, also a tumor-supressor). E7 particularly binds to Rb and histone deacetylases, resulting in activation of the E2F genes, which code for a family of transcription factors. The viral proteins E6 and E7 might be of particular interest in the development of therapeutic vaccines, since they are expressed early in viral infection.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
200ug-20CBlue IceMouse
Human papilloma virus oncoprotein E7, Type 16.
As reported
Supplied as a liquid in PBS, pH 7.2.
Recognizes HPV16 E7 protein. Species Crossreactivity: E7 protein from HPV types 16 and 18.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.