Technical Data
P3105-18
Papilloma Virus, Human, Type 16/18, E7 (hPV)
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Technical Data |
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P3105-18 |
Papilloma Virus, Human, Type 16/18, E7 (hPV) |
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Description: Human papillomavirus (HPV) is a diverse group of DNA-based viruses that infect skin and mucous membranes of humans and animals. Some HPV types are the causative agents of cervical cancer, with types 16 and 18 being particularly high-risk. The viral proteins E6 and E7 disrupt normal cell cycle regulation by interacting with p53 (a tumor-suppressing transcription factor) and Rb (retinoblastoma protein, also a tumor-supressor). E7 particularly binds to Rb and histone deacetylases, resulting in activation of the E2F genes, which code for a family of transcription factors. The viral proteins E6 and E7 might be of particular interest in the development of therapeutic vaccines, since they are expressed early in viral infection. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:20-1:200 Western Blot: 1:10-1:50 Optimal dilutions to be determined by the researcher. Recommended Pair: Pairs with P3105-19 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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