Technical Data
Papilloma Virus, Human, Type 16/18, E7 (hPV)
Human papillomavirus (HPV) is a diverse group of DNA-based viruses that infect skin and mucous membranes of humans and animals. Some HPV types are the causative agents of cervical cancer, with types 16 and 18 being particularly high-risk. The viral proteins E6 and E7 disrupt normal cell cycle regulation by interacting with p53 (a tumor-suppressing transcription factor) and Rb (retinoblastoma protein, also a tumor-supressor). E7 particularly binds to Rb and histone deacetylases, resulting in activation of the E2F genes, which code for a family of transcription factors. The viral proteins E6 and E7 might be of particular interest in the development of therapeutic vaccines, since they are expressed early in viral infection.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:20-1:200
Western Blot: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Matched Pair:
Pairs with P3105-18

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2b5E393Affinity Purified
1ml-20CBlue IceMouse
> 90% pure mouse monoclonal antibody which has been purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added.
Specific the E7 protein of HPV types 16 &18.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.