		Technical Data
P3105-23A	Papilloma Virus, Human, Type 18, E6 (hPV, Human Papillomavirus Type 18 E6, E6, Early Protein E6, HpV18gp1, Protein E6)

Description:
The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. HPV early proteins E6 and E7 are the major viral oncoproteins that regulate cell proliferation through the inactivation of p53 and Rb1 tumor suppressor proteins respectively. BF7 can be used for detection of HPV in cervical smears and biopsies and analysis of E6 expression in cell transformation studies.

Applications:
Suitable for use in RIA, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	9A281	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~0.5mg/ml

Immunogen:

Gel-purified HPV-18 E6-beta-galactosidase fusion protein. Cellular Localization: Nuclear.

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.2. No preservatives added.

Specificity:

Recognizes human HPV18 E6 at 17kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

This product has been used in:Banks L et al. Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas. J Gen Virol 68 ( Pt 5):1351-9 (1987).