		Technical Data
P3105-23B	Papilloma Virus, Human, Type 18, E6 (hPV, Human Papillomavirus Type 18 E6, E6, Early Protein E6, HpV18gp1, Protein E6)

Description:
The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. HPV early proteins E6 and E7 are the major viral oncoproteins that regulate cell proliferation through the inactivation of p53 and Rb1 tumor suppressor proteins respectively. BF7 can be used for detection of HPV in cervical smears and biopsies and analysis of E6 expression in cell transformation studies.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Formalin fixed, paraffin embedded sections. E6 levels too low for Immunofluorescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	9L397	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice		Mouse

Concentration:

~1mg/ml

Immunogen:

Gel-purified HPV 18 E6-beta galactosidase fusion protein.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.1% sodium azide.

Specificity:

Recognizes HPV 18 E6 and HPV 16 E6.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.