Technical Data
P3113-01P
PARP (Poly ADP-Ribose Polymerase)
Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry (frozen and paraffin), Immunoprecipitation, and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1,000-1:5,000
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
NSO myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F312Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human PARP.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human poly (ADP-ribose) polymerase (PARP). Species Crossreactivity: hamster, mouse, drosophila, xenopus, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Freire, R. et al. (2001) Cleavage of the Bloom's syndrome gene product during apoptosis by caspase-3 results in an impaired interaction with topoisomerase IIIalpha. Nucleic Acids Res. 29: 3172-3180. 2. Krohn, A. et al. (1998) Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector. J. Neurosci. 18: 8186 - 8197.