Suitable for use in ELISA, Immunohistochemistry (frozen and paraffin), Immunoprecipitation, and Western Blot. Other applications not tested.
Western Blot: 1:1,000-1:5,000
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.
NSO myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid, 0.09% sodium azide.|
|Recognizes human poly (ADP-ribose) polymerase (PARP). Species Crossreactivity: hamster, mouse, drosophila, xenopus, rat.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Freire, R. et al. (2001) Cleavage of the Bloom's syndrome gene product during apoptosis by caspase-3 results in an impaired interaction with topoisomerase IIIalpha. Nucleic Acids Res. 29: 3172-3180. 2. Krohn, A. et al. (1998) Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector. J. Neurosci. 18: 8186 - 8197.|