		Technical Data
P3113-01P	PARP (Poly ADP-Ribose Polymerase)

Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry (frozen and paraffin), Immunoprecipitation, and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1,000-1:5,000
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
NSO myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	10F312	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human PARP.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid, 0.09% sodium azide.

Specificity:

Recognizes human poly (ADP-ribose) polymerase (PARP). Species Crossreactivity: hamster, mouse, drosophila, xenopus, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Freire, R. et al. (2001) Cleavage of the Bloom's syndrome gene product during apoptosis by caspase-3 results in an impaired interaction with topoisomerase IIIalpha. Nucleic Acids Res. 29: 3172-3180. 2. Krohn, A. et al. (1998) Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector. J. Neurosci. 18: 8186 - 8197.