Technical Data
P3113-06A
PARP (Poly ADP-Ribose Polymerase)
Description:
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kD fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

MW of Antigen: 116kD

Epitope: N-terminal

Applications and Recommended Dilutions:
Western Blotting (Ab 5-10ug/ml for 2hrs at RT)
Immunohistochemistry (Formalin/paraffin only) (Use Ab at 6.25-12.5ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.

Positive Control: Raji cells. Tonsil

Cellular Localization: Nuclear

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide for the N-terminal region of human PARP.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See P3113-06AX
Specificity:
May be useful for the detection of poly (ADP-ribose) polymerase in cells undergoing replication or DNA repair. Species Crossreactivity: human, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tewari M, et al. Cell 81:801–809 (1995). 2. Negroni M and Bertazzoni U. Biochimica et Biophysica Acta. 1173: 133–140 (1993). 3. Lamarre D, et al. Biochimica et Biophysica Acta. 950: 147- 160 (1988)