Technical Data
P3113-06AX
PARP (Poly ADP-Ribose Polymerase) (BSA & Azide Free)
Description:
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kD fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 5-10ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin only): 6.25-12.5ug/ml for 30 min at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Raji cells, Tonsil

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide for the N-terminal region of human PARP. Molecular Weight: 116kD. Epitope: N-terminal. Cellular Localization: Nuclear.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See P3113-06A.
Specificity:
Recognizes human poly (ADP-ribose) polymerase in cells undergoing replication or DNA repair. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tewari M, et al. Cell 81:801-809 (1995). 2. Negroni M and Bertazzoni U. Biochimica et Biophysica Acta. 1173: 133-140 (1993). 3. Lamarre D, et al. Biochimica et Biophysica Acta. 950: 147-160 (1988)