Technical Data
PARP, Cleavage Site (Asp214) (Poly ADP-Ribose Polymerase)
PARP, a 116kD nuclear poly (ADPribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICElike caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP the cleavage occurs between Asp214 and Gly215, which separates PARPs N-terminal DNA binding domain (24kD) from its C-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry (IF): 1:200
Flow Cytometry: 1:25

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide (KLH coupled) corresponding to C-terminal residues adjacent to Asp214 in human PARP.
Purified by Protein A affinity chromatography.
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Recognizes endogenous levels of the large fragment (89kD) of human PARP produced by caspase cleavage. Does not react with full length PARP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356358. 2. Lazebnik, Y.A. et al. (1994) Nature 371, 346347. 3. Cohen, G.M. (1997) Biochem. J. 326, 116. 4. Nicholson, D.W. et al. (1995) Nature 376, 3743. 5. Tewari, M. et al. (1995) Cell 81, 801809. 6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 3353333539. 7. Brunet, A. et al. (2001) Mol. Cell. Biol. 21, 952965. 8. Jiang, C. et al. (2001) Cancer Res. 61, 30623070.