Technical Data
P3113-50
Parvalbumin
Description:
Parvalbumin is a calcium binding albumin protein. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is localized in fast-contracting muscles, where its levels are highest, and in the brain and some endocrine tissues. Parvalbumin is present in GABAergic interneurons in the nervous system, predominantly expressed by chandelier and basket cells in the cortex.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000 using 10ug of mouse brain lysate.
Immunocytochemistry: Cultured neurons
Immunohistochemistry: 1:1000-1:2000 (Indirect Immunoperoxidase). Works on formalin fixed, paraffin embedded tissue sections of rat cerebellum. Fixation does not appear to affect ability to detect parvalbumin.
Optimal dilution determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-14C: IgG, (HRP) Pab Gt x Mo
I1904-19C: IgG, (HRP) Pab Gt x Mo

Recommended Control:
Brain tissue, cultured neurons

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q137Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceFrogMouse
Concentration:
Not Determined
Immunogen:
Parvalbumin purified from frog muscle. (NM_002854.2)
Purity:
Ascites
Form
Supplied as a liquid, 0.1% sodium azide.
Specificity:
Recognizes frog Parvalbumin at ~12kD. Reacts with parvalbumin from brain and muscle. Directed against an epitope at the first Ca+2-binding site and specifically stains the Ca+2-bound form of parvalbumin. Species Crossreactivity: human, rat, bovine, porcine, feline, rabbit, canine, fish, mouse and goat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Perrotti, L.I., et al., J. Neurosci. 24(47): 10,594-10,602 (2004). 2. Wu, M., et al., J. Neurosci. 22: 7754-7765 (2002). 3. Bender, R.A., et al., European Journal of Neuroscience 13: 679-686 (2001).