Technical Data
P3113-50A
Parvalbumin (PVALB, Parvalbumin alpha, D22S749)
Description:
Alpha parvalbumin is a water-soluble calcium-binding protein found in select fast-spiking inhibitory GABAergic interneurons with a calcium buffering capacity. Neurons that contain parvalbumin seem to be resistant to ischaemia, epilepsy and N-methyl-D-aspartate receptor agonists due to their ability to buffer increases in intracellular albumin. Reduced expression of alpha parvalbumin has been reported in Purkinje cells in cases of spinocerebellar ataxia-1. This antibody is specific for the alpha form of parvalbumin.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:200-1:400 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Cerebellum (cytoplasmic and nuclear staining pattern)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12E11Supernatant
SizeStorageShippingSourceHost
1ml-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Recombinant fusion protein representing most of the parvalbumin alpha molecule.
Purity:
Supernatant
Form
Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
Specificity:
Recognizes Paravalbumin. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Young A N, Amin M B, Moreno C S, et al.. Expression profiling of renal epithelial neoplasms: a method for tumor classification and discovery of diag- nostic molecular markers. American Journal of Pathology. 158(5):1639-1651 (2001). 2.Brady D R and Mufson E J. Parvalbumin-immunoreactive neurons in the hippocampal formation of Alzheimer’s diseased brain. Neuroscience. 80(4):1113-1125 (1997). 3.
Vig P J S, Fratkin J D, Desaiah D, et al.. Decreased parvalbumin immunoreactivity in surviving Purkinje cells of patients with spinocerebellar ataxia-1. Neurology 47:249-253 (1996). 4.Sorvari H, Soininen H, Paljärvi L, et al.. Distribution of parvalbumin-immunoreactive cells and fibers in the human amygdaloid complex. The Journal of Comparative Neurology. 360:185-212 (1995). 5.Föhr U G, Weber B R, Müntener M, et al.. Human a+and b parvalbumins, structure and tissue-specific expression. European Journal of Biochemistry. 215:719-727 (1993).