Technical Data
P3113-51
Parvalbumin
Description:
Calbindins are Ca-binding proteins belonging to the troponin C superfamily. There are two types of CaBPs: the "trigger"- and the "buffer"-CaBPs. The conformation of "trigger" type CaBPs changes upon Ca2+ binding and exposes regions on protein that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. Parvalbumin (PARV, PVALB or oncomodulin) is a high affinity low mol wt (~12kD) cytoplasmic protein belonging to the troponin C superfamily. It is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. PARV has two isoforms: alpha (rat/mouse/human: 110aa, 7p13-p11) and beta form (mouse/rat/human: 109aa) shares ~48% identity (mouse PARVs). The PARV-alpha is less acidic (pI=5) than the beta isoform and has an additional amino acid in the C-terminal helix. PARV-alpha is also expressed in inner ear. PARV-beta isoform is expressed in many fetal tissues, placenta and tumors.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:100,000. In ELISA, antibody reactivity is Calcium ion dependent.
Western Blot: 1:2000-5000 (ECL). MW: ~12kD.
Immunohistochemistry & Immunofluorescence: 1:500-1:1000; Indirect immunoperoxidase labeling of formalin-fixed, paraffin embedded sections of rat brain.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H43Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceFrogMouse
Concentration:
Not determined
Immunogen:
Purified frog muscle Parvalbumin
Purity:
Ascites
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes frog Parvalbumin. Crossreactivity with respect to the isoforms (PARV-alpha and beta) has not been studied. No significant crossreactivity is seen with other CABPs or EF-hand containing proteins. Species Crossreactivity: bovine, human, mouse, rat, canine, feline and fish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Berchtold, M.W., et al., JBC 262: 8696-8701 (1987). 2. Ritzler, J.M., et al., Genomics 12: 567-572 (1992). 3. Schoffl, F., et al., Intl. J. Biochem. 22: 1211-1215 (1990). 4. Wahr, P.A., et al., PNAS 96: 11982-11985 (1999). 5. Zuhlke, C., et al., Genet. Res. 54: 37-43 (1989).