Technical Data
P3113-51B
Parvalbumin (PVALB, D22S749, HGNC:9704, MGC116759, Parvalbumin alpha)
Description:
Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1ug/ml. Detects a band of ~12kD, representing parvalbumin from rat cerebellum. Since this protein is relatively small, it is recommended that electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
Immunoprecipitation: 5ug/ml
Immunohistochemistry (Paraffin): 1ug/ml
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Purified parvalbumin from rat skeletal muscle. Cellular Localization: Cytoplasmic and Nuclear
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 15mg/ml BSA, 0.05% sodium azide.
Specificity:
Recognizes rat Parvalbumin. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Xiong H, Xia K, Li B, et al. KChIP1: a potential modulator to GABAergic system. 2009:gmp013