		Technical Data
P3113-85C	Paxillin

Description:
The cytoskeletal adaptor protein Paxillin is found at the interface between actin filaments and the
plasma membrane. Paxillin localizes to focal adhesions, where it provides a platform for the
integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation is required for integrin-mediated cytoskeletal reorganization, and may play a role
in the disassembly of focal adhesions and stress fibers during cellular transformation. Increased
Paxillin protein levels have also been reported in several malignancies, including renal, lung, and
breast carcinomas.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunocytochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	9H169	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~500ug/ml

Immunogen:

Recombinant human Paxillin, aa59-274; P49023 (E. coli).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.

Specificity:

Recognizes human Paxillin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.