Technical Data
P3113-85G
Paxillin
Description:
Paxillin is a multi-domain protein that localizes primarily to focal adhesion sites to the extracellular matrix (1). Paxillin is one of the key components of integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at tyrosine 118 (3,4). Phospho-Tyr118 may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic non-phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-YP-S-F)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of total human Paxillin protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Turner, C.E. (2000) J. Cell Sci. 113, 41394140. 2. Burridge, K. et al. (1992) J. Cell Biol. 119, 893903. 3. Bellis, S.L. et al. (1995) J. Biol. Chem. 270, 1743717441. 4. Bellis, S.L. et al. (1997) Biochem. J. 325, 375381. 5. Schaller, M.D. and Parsons, J.T. (1995) Mol. Cell. Biol. 15, 26352645.