Technical Data
Paxillin, phosphorylated (Tyr118)
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyr118 phosphorylation of paxillin occurs in response to focal adhesion kinase (FAK).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
L6 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul4C/-20CBlue IceHumanRabbit
Synthetic peptide corresponding to human Paxillin phosphorylated at Tyr118 (KLH).
Purified by immunoaffinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Recognizes human Paxillin, phosphorylated at (Tyr118). Species Crossreactiviy: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.