Technical Data
P3113-85N
Paxillin, phosphorylated (Tyr31) (PXN)
Description:
Paxillin is a 65kD cytoskeletal adaptor protein and member of the Paxillin family. Human Paxillin is 591aa in length and contains four LIM zinc-binding domains. Alternative splicing produces three isoforms. Human Paxillin shares 94% and 85% aa identity with mouse and rat Paxillin, respectively. Paxillin is found at the interface between actin filaments and the plasma membrane, and it localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion-and growth factor-related signals. Paxillin phosphorylation at tyrosines 31 and 118 is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Immunocytochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2A12K157Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml after reconstitution
Immunogen:
Synthetic phosphopeptide corresponding to human Paxillin Tyr31 site.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Specificity:
Recognizes human Paxillin when phosphorylated at Tyr31.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.