Technical Data
P3113-86
Paxillin (PXN)
Description:
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation (Use Protein G): 2ug/mg protein lysate; Native verified
Western Blot: 1-2ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control: HeLa cells and Colon carcinoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3F312Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Puri ed recombinant full-length human paxillin protein. Cellular Localization: Cell membrane and cytoplasmic MW of Antigen: 68kD
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See P3113-86X.
Specificity:
Recognizes human Paxillin. Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. de Curtis, I. and Malanchini, B., Exp. Cell Res. 230: 233–243 (1997). 2. Hanks, S.K. and Polte, T.R., Bioessays 19(2): 137–145 (1997). 3. Leventhal, P.S., et al., J. Biol. Chem. 272(8): 5214–5218 (1997).