Technical Data
P3113-86X
Paxillin (BSA & Azide Free) (PXN)
Description:
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

MW of Antigen: 68kD

Epitope: Not determined

Applications and Recommended Dilutions:
Immunofluorescence
Immunoprecipitation (Native verified)
(Use Protein G) (Ab 2ug/mg protein lysate)
Western Blotting (Ab 1-2ug/ml for 2hrs at RT))
Immunohistochemistry (Formalin/paraffin) (Ab 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.

Positive Control: HeLa cells. Colon carcinoma.

Cellular Localization: Cell membrane and cytoplasmic

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20C or colder. Aliquots are stable for at least 12 months at-20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k3F312Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Puri ed recombinant full-length human paxillin protein
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See P3113-86
Specificity:
Recognizes Paxillin. Species Crossreactivity: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. de Curtis I; Malanchini B. Exp Cell Res, 1997, 230:23343. 2. Hanks SK; Polte TR. Bioessays, 1997 Feb, 19(2):13745. 3. Leventhal PS; et al. J Biol Chem, 1997, 272(8):52148.