Technical Data
P3113-86X
Paxillin (BSA & Azide Free) (PXN)
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Technical Data |
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P3113-86X |
Paxillin (BSA & Azide Free) (PXN) |
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Description: Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation. MW of Antigen: 68kD Epitope: Not determined Applications and Recommended Dilutions: Immunofluorescence Immunoprecipitation (Native verified) (Use Protein G) (Ab 2ug/mg protein lysate) Western Blotting (Ab 1-2ug/ml for 2hrs at RT)) Immunohistochemistry (Formalin/paraffin) (Ab 1-2ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. Positive Control: HeLa cells. Colon carcinoma. Cellular Localization: Cell membrane and cytoplasmic Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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