Technical Data
Paxillin (PXN)
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of proteinprotein interactions with kinases, GTPase-activating
proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growthfactor activation and fibronectins. Both JNK1 and cdc2kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.

Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG15i46Affinity Purified
100ul-20CBlue IceHumanMouse
Full-length recombinant human paxillin.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Detects a 68kD protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells. Similar results were seen in various cells and tissues from rat, mouse, and chicken origin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
2003 Woodrow, M.A. Exp. Cell. Res. 287(2):325-338.
2003 Huang, C. et al. Nature 424:219-223.
2004 Huang, C. et al. Cell Cycle 3(1):4-6.