Technical Data
Paxillin, phosphorylated (Ser17)
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding
domains that participate in a variety of protein-protein interactions with kinases, GTPase-
activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both
tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-
factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine
178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes
and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during
cell migration.

Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500
ELISA: 1:2000
Optimal dilution to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Detects a 68kD protein corresponding to the molecular weight of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic endothelial cells. Epitope: phosphoserine 178. Species Cross Reactivity: Human. This human sequence is highly conserved in rat and mouse paxillin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
RELATED REFERENCE: 1. Huang, C. et al. (2003) Nature 424:219-223. Huang, C. et al. (2004) Cell Cycle 3(1):4-6. 2. Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.